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Literature summary extracted from

  • Takabe, P.; Bart, G.; Ropponen, A.; Rilla, K.; Tammi, M.; Tammi, R.; Pasonen-Seppaenen, S.
    Hyaluronan synthase 3 (HAS3) overexpression downregulates MV3 melanoma cell proliferation, migration and adhesion (2015), Exp. Cell Res., 337, 1-15.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 isozyme HAS3 inducible overexpression in MV3/C8161 cells. EGFP-HAS3 overexpression rapidly induced pericellular hyaluronan coat formation and plasma membrane protrusions, induction by doxycycline Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.212 additional information isozyme HAS3 overexpression expands the cell surface hyaluronan coat and decreases melanoma cell adhesion, migration and proliferation by cell cycle arrest at G1/G0. Melanoma cell migration is restored by removal of cell surface hyaluronan by Streptomyces hyaluronidase and by receptor blocking with hyaluronan oligosaccharides, while the effect on cell proliferation is receptor independent. Overexpression of isozyme HAS3 decreases ERK1/2 phosphorylation suggesting that inhibition of MAP-kinase signaling is responsible for the suppressive effects on the malignant phenotype of MV3 melanoma cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.212 plasma membrane
-
 Homo sapiens 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 skin
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 Has3
-
 Homo sapiens
2.4.1.212 hyaluronan synthase 3
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.1.212 Homo sapiens doxycycline treatment causes a strong upregulation of HAS3 expression. A distinct EGFP-HAS3 signal is observed already 2 h after starting the doxycycline induction, localized mainly at the plasma membrane and in the Golgi area. After 24 h induction EGFP-HAS3 signal has become more intense, localizing strongly at the Golgi area and at the plasma membrane up

General Information

EC Number General Information Comment Organism
2.4.1.212 malfunction isozyme HAS3 overexpression downregulates MV3 melanoma cell proliferation, migration and adhesion. Overexpression of isozyme HAS3 decreases cell proliferation, directional and random cell migration, and promotes cell cycle arrest at G1/G0 and decreases ERK1/2 phosphorylation suggesting that inhibition of MAP-kinase signaling is responsible for the suppressive effects on the malignant phenotype of MV3 melanoma cells. EGFP-HAS3 overexpression downregulates several signaling pathways in MV3 melanoma cells Homo sapiens
2.4.1.212 physiological function the enzyme is involved in synthesis of hyaluronan that may have anti-cancer like effects in melanoma progression Homo sapiens